Journal: ACS Omega

Article Title: Click Chemistry-Generated Auristatin F–Linker–Benzylguanine for a SNAP-Tag-Based Recombinant Antibody–Drug Conjugate Demonstrating Selective Cytotoxicity toward EGFR-Overexpressing Tumor Cells

doi: 10.1021/acsomega.2c06844

Figure Lengend Snippet: Live cell imaging of 1711(scFv)-SNAP-Alexa Fluor 647 binding to EGFR-positive cells. (A–D) Confocal microscopy images of 1711(scFv)-SNAP-Alexa Fluor 647 binding and uptake (red) in target cell lines after 30 min of incubation at room temperature. Hoechst was used as a stain for the nuclei (blue). (A) Surface binding of 1711(scFv)-SNAP-Alexa Fluor 647 to A431 cells at 37 °C. (B) Specific surface binding and internalization of 1711(scFv)-SNAP-Alexa Fluor 647 in MDA-MB-468 cells at room temperature. (C) No binding of 1711(scFv)-SNAP-Alexa Fluor 647 to the EGFR-negative A2058 cells. (D) Vehicle control with MDA-MB-468 cells incubated in 1× PBS at room temperature.

Article Snippet: MDA-MB-468 (ATCC: HTB-132), A431 (ATCC: CRL-259), and A2058 (ATCC: CRL-11147) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (containing 2 mM L-glutamine, 3.7 g/L C 3 H 3 NaO 3 and 16 mg/L phenol red) supplemented as above.

Techniques: Live Cell Imaging, Binding Assay, Confocal Microscopy, Incubation, Staining, Control

Journal: International Journal of Nanomedicine

Article Title: Prior anti-CAFs break down the CAFs barrier and improve accumulation of docetaxel micelles in tumor

doi: 10.2147/IJN.S171224

Figure Lengend Snippet: Distribution and retention of micelles in 4T1 tumor bearing mice. Notes: ( A ) IVIS and ( B ) radiant efficiency analysis of DiR-Ms after different treatments (n=3). * P <0.05, ** P <0.01. Abbreviations: IVIS, in vivo imaging systems; TC therapy, two-stage combination therapy; CC therapy, co-dose combination therapy; DTX-Ms, docetaxel micelles; DiR-Ms, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide-loaded micelles.

Article Snippet: The radiant efficiencies at the tumor site were analyzed with IVIS spectrum system software (Living Image 4.3.1, PerkinElmer Inc., Waltham, MA, USA).

Techniques: In Vivo Imaging